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The metalloendopeptidase meprin - degradomic approaches: Principles and applications in protease substrate discovery



By: Daniel Ambort(Author)

Language: German

Genre: Science & Nature

Publisher: VDM Verlag (1 April 2009)

Format: pdf doc docx mobi djvu epub ibooks (*An electronic version of a printed book that can be read on a computer or handheld device designed specifically for this purpose.)

The original title of the book: The metalloendopeptidase meprin - degradomic approaches: Principles and applications in protease substrate discovery

In the past, singly-selected protease-substrates (proteins cleaved by enzymes) were tested by invitro cleavage assays and thus substrate discoverywas haphazard. Nowadays, with the rapidly growingfield of proteomics (study of entire protein sub- sets of a cell), a protease and its substraterepertoire (degradome) may be studied in a complexcontext to identify physiologically relevantsubstrates. The author Daniel Ambort describes step- by-step first the principles of degradomics and thenits application in protease-substrate discovery. Twobiological systems, a cell-based (culture medium- derived proteins) and a cell-free (human milk aspolysubstrate), were used to find hitherto unkownsubstrates for the metalloendopeptidase meprin (zinc- dependent protein-cleaving enzyme). One- and two- dimensional gel electrophoresis together with massspectrometry enabled the identification of novelmeprin cleavage products. In summary, this bookcombines standard textbook knowledge with laboratory- intense hard work and it thus encompasses a completedescription of the realization of degradomicsprojects.

Daniel Ambort, PhD: Studies in Biology andBiochemistry at the Dept. of Chemistry and Biochemistry,University of Berne, Switzerland. Dissertation inBiochemistry at IBMM, University of Berne, Switzerland. Post-docin the Mucin Biology Group at the Dept. ofMedical Biochemistry, University of Gothenburg, Sweden.

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